Methods and compositions for production of recombinant protein in HBX-expressing mammalian cells

摘要

The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consisting of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method.

主权项

1. A method of producing a heterologous protein in mammalian host cells in culture, comprising growing mammalian host cells selected from the group consisting of HKB11 cells, CHO cells, BHK21 cells, C2C12 cells, and HEK293 cells, wherein said mammalian host cells comprise a nucleic acid encoding HBx, a nucleic acid encoding the heterologous protein, and a nucleic acid providing exogenous XBP1s, wherein the nucleic acid providing exogenous XBP1s encodes XBP1 or XBP1s, under conditions such that HBx, exogenous XBP1s and the heterologous protein are expressed by the mammalian host cells.