Culture substrate for human pluripotent stem cells and use thereof

摘要

The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin α5β1γ1 E8 fragment or human laminin α3β3γ2 E8 fragment preferably at a concentration of 0.5 to 25 μg/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.

主权项

1. A culture method of human pluripotent stem cells dissociated into single cells in feeder-free culture conditions, comprising the steps of:
dissociating human pluripotent stem cells into single cells, and culturing the human pluripotent stem cells dissociated into single cells using a culture substrate, the culture substrate being coated with human laminin β5β2γ1 E8 fragment or human laminin α3β3γ2 E8 fragment.