APPARATUS AND METHOD FOR AN INCLINED SINGLE PLANE IMAGING MICROSCOPE BOX (ISPIM BOX)

摘要

An apparatus for inclined single plane Illumination microscopy of a sample includes a laser for launching excitation light beams at a plurality of wavelengths, a laser beam expander, an injection arm optically coupled to the laser beam expander, a conventional back-to-back microscope system, a universal dichroic mirror optically coupled to the injection arm to direct the excitation light beams into the conventional back-to-back microscope onto a sample plane in an imaging plane, and to receive fluorescence light from the sample, a universal optical adaptor optically coupled to the universal dichroic mirror, a re-imaging component optically coupled to the universal optical adaptor; and a camera output connector optically coupled to the re-imaging component, where the laser beam expander, injection arm, universal optical adapter, re-imaging component, and camera are combined in a modular unit which is arranged and configured to be coupled to the conventional back-to-back microscope.

主权项

1. An apparatus for inclined single plane Illumination microscopy of a sample comprising:
a laser for launching excitation light beams at a plurality of wavelengths; a laser beam expander for expanding the excitation light beams optically; an injection arm optically coupled to the laser beam expander; a conventional back-to-back microscope system having an first excitation objective and a second collection objective; a universal dichroic mirror optically coupled to the injection arm to direct the excitation light beams into the conventional back-to-back microscope, the first excitation objective onto a sample plane in an imaging plane, and to receive from the conventional back-to-back microscope objective fluorescence light from the sample; a universal optical adaptor having a second collection objective and a third collection objective optically coupled to the universal dichroic mirror; a re-imaging component optically coupled to the universal optical adaptor; and a camera optically coupled to the re-imaging component, where the laser beam expander, injection arm, universal optical adapter, re-imaging component, and camera are combined in a modular unit which is arranged and configured to be coupled to the conventional back-to-back microscope and without perpendicularity between the second and third objectives.