METHODS AND SYSTEMS FOR THE COLLECTION OF LIGHT USING TOTAL INTERNAL REFLECTANCE

摘要

Aspects of the present disclosure include a flow cell nozzle configured to propagate light emitted by a sample in a flow stream upstream by total internal reflectance. Flow cell nozzles according to certain embodiments include a nozzle chamber having a proximal end and a distal end and a nozzle orifice positioned at the distal end of the nozzle chamber where the flow cell nozzle is configured to propagate light emitted from a sample in the flow stream upstream through the flow cell nozzle orifice by total internal reflectance toward the proximal end of the nozzle chamber. Systems and methods employing the subject flow cell nozzles are also provided.