Transformation system in the field of filamentous fungal hosts

摘要

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

主权项

1. A modified cell of a fungal strain derived from Chrysosporium lucknowense strain C1 (VKM F-3500 D), now classified as Myceliopthora thermophilum C1, comprising at least one nucleic acid sequence encoding one or more polypeptides,
wherein at least one nucleic acid sequence encoding one or more polypeptides is operably linked to one or more promoters operable in a fungal cell; wherein zero or more of said nucleic acid sequences encoding one or more polypeptides comprise a sequence encoding a signal peptide fused in frame to a sequence encoding a polypeptide; wherein said modified strain expresses one or more of said polypeptides at a higher level than a corresponding parental or non-modified strain under the same culture conditions.