| 摘要 |
The present invention provides a novel endo-β-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-β-N-acetylglucosaminidase (Endo-Om) gene originated from a methylotrophic yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including complex type sugar chains, in glycoproteins and the modification of the sugar chains. Also provided are an endo-β-N-acetylglucosaminidase (Endo-Cp), an endo-β-N-acetylglucosaminidase (Endo-Pa) and an endo-β-N-acetylglucosamimidase (Endo Zr) which are produced from Candida parapolymorpha DL-1 ATCC26012 strain, Pichia anomala ATCC36904 strain and Zygosaccharomyces rouxii ATCC2623 strain, respectively, on the basis of an Endo-Om gene sequence, and each of which has a similar level of complex type sugar chain cleavage activity and a similar level of complex type sugar chain transfer activity to those of Endo-Om. |
