Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification

摘要

A method of simultaneously co-purifying and concentrating nucleic acid and protein targets is described. The method includes automation of the entire sample preparation process, performed by having an analyst add a sample into a device that performs all of the steps necessary to prepare a sample for analysis. The method provides for samples that are not split during the sample preparation process and where common purification methods can be used for purifying multiple analytes.

主权项

1. A method of simultaneously separating and concentrating nucleic acid and protein targets, the steps comprising:
(i) obtaining a sample comprising a nucleic acid and a protein; (ii) adding said sample to a gel electrophoresis unit, said gel electrophoresis includes a gel box with a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl) methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than said first buffer; (iii) subsequent to step (ii), subjecting said sample to isotachophoresis, said subjecting step comprising a step of applying a voltage to said gel electrophoresis unit; and (iii) subsequent to step (iii), detecting the presence of the nucleic acid and the protein.