RECOMBINANT PLASMID DNA pQE30/derf2L CODING PROTEIN der f 2L OF MITE Dermatophagoides farinae, AND BACTERIAL STRAIN Escherichia coli M15/pQE30/derf2L - PRODUCER OF SUCH PROTEIN

摘要

FIELD: medicine.SUBSTANCE: inventions refer to biotechnology and concern a recombinant plasmid DNA pQE30/Derf2L and a bacterial strain Escherichia coli M15/pQE30/Derf2L. The characterised recombinant plasmid DNA codes protein Derf2L of a mite Dermatophagoides farinae and consists of BamHI/HindIII - a DNA fragment of a plasmid pQE30 and a DNA sequence coding BamHI/HindIII fragment including gene Derf2L Dermatophagoides farinae. The characterised bacterial strain Escherichia coli M15/pQE30/Derf2L, is produced by transformation of the cells Escherichia coli M15 by the recombinant plasmid DNA pQE30/Derf2L.EFFECT: presented inventions can be applicable to develop a dust mite diagnosticum, as well as for conducting specific immune therapy.2 cl, 3 dwg, 1 tbl, 3 ex