METHOD OF PRODUCING HETEROGENEOUS PROTEIN

摘要

The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.

主权项

1. A method of producing an antibody, comprising culturing an animal cell which expresses alanine aminotransferase and has a transferred DNA encoding the antibody and thereby allowing the animal cell to produce the antibody,
wherein the animal cell is a cell transformed with DNA encoding the alanine aminotransferase; and wherein the alanine aminotransferase is any one of the following (a)-(c): (a) a polypeptide having the amino acid sequence as shown in SEQ ID NO: 9; (b) a polypeptide which has an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 9 by substitution, deletion, addition and/or insertion of 1-5 amino acid residues and yet has alanine aminotransferase activity; and (c) a polypeptide having 99% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO: 9 and yet having alanine aminotransferase activity.