Method for identification and detection of mutant gene using intercalator

摘要

The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant DNA and a probe and/or a hybrid of a wild type DNA and a probe, said hybrids being obtained by the LH method. A method for the detection of a mutant DNA and/or a wild type DNA, said method comprising: step (1) for bringing into contact at least one kind of a single-stranded DNA (mutant DNA) having a substituted base, a deleted base region or an inserted base region, and/or a corresponding wild type single-stranded DNA (wild type DNA), with a probe that is hybridizable with both of the single stranded DNAs to form a hybrid (mutant hybrid) with the mutant DNA and/or a hybrid (wild type hybrid) with the wild type DNA, provided that the obtained mutant hybrid and/or wild-type hybrid have a loop structure; step (2) for bringing into contact the obtained mutant hybrid and/or wild type hybrid with an intercalator; and step (3) for separating a conjugate of the mutant hybrid with the intercalator and/or the conjugate of the wild-type hybrid with the intercalator to thereby detect the presence or absence of the mutant DNA and/or the wild type DNA.