Modified oligonucleotides comprising thiol functions and the use of same for the detection of nucleic acids

摘要

The present invention concerns a modified oligonucleotide having two or a plurality of thiol functions, capable of being immobilised on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or one carbon-carbon triple bond or halogen acetamide functions, preferably maleimide or acrylamide functions. The invention also concerns a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridisation between a modified oligonucleotide and an amplified target nucleic acid from the biological sample. The invention more specifically concerns a method for detecting genotyping or sequencing of a pathogenic organism, preferably a virus.