A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte; (2) producing captured molecules by reacting each single nucleotide base with a capture system; (3) amplifying at least part of the captured molecule to produce a plurality of amplicons characteristic of the single nucleotide base; (4) labelling the amplicons with a corresponding probe having a characteristic detectable element and (5) detecting a property characteristic of the detectable element.
申请公布号
CA2907868(A1)
申请公布日期
2014.10.16
申请号
CA20142907868
申请日期
2014.04.09
申请人
BASE4 INNOVATION LTD.;MEDICAL RESEARCH COUNCIL
发明人
FRAYLING, CAMERON ALEXANDER;BALMFORTH, BARNABY;SOARES, BRUNO FLAVIO NOGUEIRA DE SOUSA;ISAAC, THOMAS HENRY;BREINER, BORIS;NATALE, ALESSANDRA;AMASIO, MICHELE;DEAR, PAUL