摘要 |
<p> The purpose of the present invention is to provide a method that can accurately detect DNA having a microsatellite region without causing the problem of non-specific reaction products. The present invention relates to a "method for detecting DNA having a microsatellite region by (1) bringing a probe, which does not have a nucleotide sequence complementary to the microsatellite region and which will hybridize with the nucleotide sequences on both sides of the microsatellite region, into contact with DNA having the microsatellite region, and forming a hybrid of the probe and the DNA, said hybrid having a loop structure containing the microsatellite region, (2) separating the obtained hybrid, and (3) detecting the hybrid", and a "hybrid of a probe and DNA, said hybrid having a loop structure containing a microsatellite region and obtained by bringing a probe, which does not have a nucleotide sequence complementary to the microsatellite region and which will hybridize with the nucleotide sequences on both sides of the microsatellite region, into contact with DNA having the microsatellite region".</p> |