In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells

摘要

A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

主权项

1. A method of producing Chromogranin A (CHGA)-negative cells comprising, contacting human definitive endoderm lineage cells in vitro with a TGFβ superfamily member and a Wnt family member thereby producing CHGA-negative cells.