| 主权项 |
1. A microinjection method applied to a microinjection device that includes an injecting member having a hollow point portion and introduces a substance contained in the injecting member to a solution including endoplasmic reticulum, the microinjection method comprising:
measuring a correlation, for a first solution, between fluorescence intensity and injection amount, the measuring including:
injecting the first solution containing a fluorescent reagent into a second solution that forms an interface with the first solution through the injecting member to form a droplet of the first solution;irradiating the first solution with excitation light;measuring a diameter of the droplet from an image of the droplet;detecting fluorescence intensity of the droplet; andobtaining the measured correlation between fluorescence intensity and injection amount from the measured diameter of the droplet and the detected fluorescence intensity; injecting the first solution into a third solution that does not form an interface with the first solution through the injecting member, and measuring pressure and pressurizing time; irradiating the third solution including the first solution with excitation light; detecting fluorescence intensity of the first solution included in the third solution; calculating an injection amount of the first solution injected into the third solution from the detected fluorescence intensity and the measured correlation; obtaining a correlation between an injection amount of the first solution from the injecting member, pressure, and pressurizing time based on the measured pressure and pressurizing time and the calculated injection amount; and adjusting an amount of the first solution injected into the third solution including the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation between the injection amount, the pressure, and the pressurizing time. |
