Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate

摘要

The object of the present invention is to provide a method for immobilizing the SpA protein on the surface of a substrate with high density without causing dimerization.;The following method solves the object. That is, the method for binding a protein to a surface of a substrate, comprising steps (A) to (B): step (A) of preparing said protein to the surface, step (B) of supplying said protein to the surface, wherein said protein consists of a Protein A or at least one domain of A to E of said Protein A, and said protein comprises C-terminal modified amino acid sequence represented by SEQ ID:1(SFNRSEC).

主权项

1. A method for binding a protein to a surface of a substrate, the method comprising the following steps (A) to (C):
(A) preparing said substrate with the surface represented by the following chemical formula: wherein n represents an integer within the range of 2 to 20; (B) supplying a solution containing said protein to the surface, wherein said protein consists of a D domain of a Protein A, and a C-terminal of said D domain of said Protein A is linked to the serine residue of an amino acid sequence represented by SEQ ID NO:1(SFNRNEC); and (C) binding the cysteine residue of the amino acid sequence represented by SEQ ID NO:1 to the surface of said substrate.