CARBONIC ANHYDRASE IX-RELATED MARKERS AND USE THEREOF

摘要

The present disclosure provides methods for detecting the presence of a cancer stem cell and their use in cancer prognosis, evaluating risk of cancer metastasis, identifying or validating drug candidates, and determining treatment efficacy. It also provides kits useful for detecting the presence of cancer stem cells as well as methods of treating cancer using CAIX inhibitors.

主权项

1. A method for identifying or validating a putative cancer therapeutic, comprising:
(a) exposing cancer cells to a putative cancer therapeutic to obtain treated cancer cells, (b) measuring, of the treated cells, levels of at least three markers, wherein each of the at least three markers is from a different type of markers, the different types of markers being selected from:
(1) cancer stem cell markers,(2) epithelial-mesenchymal transition (EMT) markers selected from mesenchymal markers and epithelial markers,(3) stemness markers,(4) mTORC1 markers, and(5) chemokine markers; (c) comparing the level of each of the at least markers measured in step (b) with a reference level for each of the at least three markers; wherein a decrease in the levels of cancer stem cell markers, mesenchymal markers, stemness markers, mTORC1 markers, and chemokine markers if measured in step (b) and an increase in the levels of epithelial markers if measured in step (b) indicate that the putative cancer therapeutic is effective in treating cancer.