| 摘要 |
Step S1: acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. Step S2: setting analysis areas to the acquired fluorescent images. Step S3: calculating a classification value of the analysis areas R2. Step S4: classifying the images into one or more groups on the basis of the classification value. Step S5: calculating an average image of the analysis area every group. Step S6: subtracting the average image from each image of the analysis area every group to calculate a new image of the analysis area. Step S7: calculating a correlation value on the basis of the new images every group. Step S8: performing estimation of a diffusion time or diffusion constant, a molecule number, etc. by use of a fitting equation. |
