Immunodetection and quantification of pyrazolopyrimidine sedatives

摘要

The invention relates to novel immunogens, antibodies, methods and kits for use in immunoassays to detect and quantify zaleplon, metabolites of zaleplon and indiplon. These are the first described immunoassays for these compounds and have greater sensitivity than alternative analytical techniques.

主权项

1. A method of detecting or determining an amount of zaleplon in a solution or an in vitro sample taken from an individual, the method comprising:
(i) contacting the sample or the solution with a conjugate and an antibody raised against an immunogen of the structure: wherein n=0 or 1, the accm is an antigenicity-conferring carrier material and the crosslinker links a carbonyl group to the accm; (ii) detecting the bound conjugate, wherein the conjugate comprises coupled to a detectable labelling agent, wherein the detectable labelling agent is coupled to the carboxylic acid group of Hapten A; and (iii) deducing from calibration value(s) the presence of an amount of zaleplon.