摘要 |
Disclosed is a method for the isomerisation of glucose by reduction to sorbitol and subsequent oxidation into fructose, in which the redox cofactors NAD+/NADH and NADP+/NADPH are regenerated in a one-pot reaction, wherein one of the two redox cofactors is obtained in the reduced form thereof while the other redox cofactor is obtained in the oxidized form thereof as a result of at least two additional enzymatically catalysed redox reactions (product forming reactions) taking place in the same reaction batch, wherein (a) in the regeneration reaction, which transfers the reduced cofactor back into its originally oxidised form, oxygen or a compound of the general formula R1C(O)COOH is reduced, and (b) in the regeneration reaction, which transfers the oxidised cofactor back into its originally reduced form, a compound of the general formula R2CH(OH)R3 is oxidised, wherein R1, R2 und R3 have different meanings in the compounds, characterised in that a mixture of glucose and fructose is used as a starting material and by the use of fructose thus produced in a method for producing furan derivatives. |