METHOD TO CONSTRUCT IN-VITRO HUMAN BLOOD BRAIN BARRIER MODEL

摘要

A method to construct an in-vitro human blood brain barrier (BBB) model is disclosed, which comprises steps: attaching suspension liquids of human brain vascular pericytes (HBVPs) and human astrocytes (HAs) by a ratio of 1:1, 1:2, or 1:6 to a bottom surface of a filter membrane of a culture dish to plant HBVPs and HAs on the bottom surface; filling a suspension liquid of human brain microvascular endothelial cells (HBMECs) into a top surface of the filter membrane to plant HBMECs on the top surface; placing the culture dish in a well plate containing a culture medium, and placing the well plate in a carbon-dioxide incubator; replacing the culture medium with a condition medium; and replacing the condition medium once daily for a plurality of days. Thereby is constructed an in-vitro human BBB model of high medical research availability.

主权项

1. A method to construct an in-vitro human blood brain barrier model, comprising steps:
attaching suspension liquids of human brain vascular pericytes (HBVPs) and human astrocytes (HAs) by a ratio of 1:1, 1:2, or 1:6 to a bottom surface of a filter membrane of a culture dish to plant said HBVPs and said HAs on said bottom surface; filling a suspension liquid of human brain microvascular endothelial cells (HBMECs) into a top surface of said filter membrane to plant said HBMECs on said top surface; placing said culture dish in a well plate containing a culture medium, and placing said well plate in a carbon-dioxide incubator; replacing said culture medium with a condition medium; and replacing said condition medium once daily for a plurality of days.