发明名称 |
METHOD OF CRYOPRESERVATION OF TISSUE DERIVED FROM PLURIPOTENT STEM CELLS |
摘要 |
<p>The present invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the following (1) to (3):
(1) a first step of bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol,
(2) a second step of maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and,
(3) a third step of cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution in the second step. According to the method of the present invention, a stable preservation method of a tissue derived from a pluripotent stem cell can be provided.</p> |
申请公布号 |
EP2784154(A1) |
申请公布日期 |
2014.10.01 |
申请号 |
EP20120851379 |
申请日期 |
2012.11.22 |
申请人 |
SUMITOMO CHEMICAL COMPANY, LIMITED;RIKEN |
发明人 |
ANDO, SATOSHI;NAKANO, TOKUSHIGE;SASAI, YOSHIKI;EIRAKU, MOTOTSUGU |
分类号 |
C12N5/074;A01N1/02;C12N1/04;C12N5/0735;C12N5/0775;C12N5/0789;C12N5/0797 |
主分类号 |
C12N5/074 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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