METHOD OF CORRECTION OF PARTICLE INTERFERENCE TO HEMOGLOBIN MEASUREMENT
摘要
<p>A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.</p>
申请公布号
EP2356465(B1)
申请公布日期
2014.10.01
申请号
EP20090826666
申请日期
2009.11.11
申请人
BECKMAN COULTER, INC.
发明人
ZHANG, SHULIANG;LU, JIULIU;ZHENG, MIN;GRACE, ERIC, M.;LI, JING;LAVERNIA, MARITZA;BRITTON, TED, W.