High throughput fabrication of nanoparticles

摘要

This application provides a high throughput method of making nanoparticles that utilizes plates comprising wells (e.g., 96-well plates).

主权项

1. A method of preparing nanoparticles comprising:
(a) providing:
(i) a first stock solution comprising an active agent and polymers admixed with one or more organic solvents;(ii) a second stock solution comprising a surfactant and a solvent in aqueous phase;(iii) a third stock solution comprising a water or buffer quench solution; and(iv) a fourth stock solution comprising an aqueous carrier and beta-cyclodextrin; (b) combining a volume of said first stock solution and a volume of said second stock solution to form an emulsification solution; (c) emulsifying said emulsification solution; (d) combining the emulsion formed in step (c) with a volume of the third stock solution; (e) adding a volume of the fourth stock solution to the solution formed in step (d); and separating beta-cyclodextrin and dissolved unencapsulated active agent from the resulting solution; and (f) collecting the nanoparticles from the solution formed in step (e).