FIBRIN SEALANT (FIBRINGLURAAS) CONSISTING OF A KIT OF LYOPHILIZED OR FROZEN HIGH CONCENTRATE FRIBINOGEN

摘要

The application is directed to a fibrin sealant (FIBRINGLURAAS®) consisting of a kit of lyophilized or frozen high concentrate fribinogen in which 5% a1at will be added into the final bulk and or 5% a1at as a diluent for high concentrate fibrinogen and new found proteins kh30, kh31, kh32, kh44, kh46, kh47, and kh52 in which the kh good healthy cells are present, either non-heated or heating to at least 1° C. and above, preferably at least 101° C., and lyophilized or frozen thrombin used to compound glue membrane, the diameter of which is less than 10 micrometers the actual size of the glue membrane of the fibrin sealant (FIBRINGLURAAS®) is from 0.6 μm, to 101° C. heating 0.005 micrometers and its topical applications for all solid tumor cancer

主权项

1. A method of purifying fibrinogen from plasma Fraction I or from plasma cryoprecipate comprising:
a) collecting plasma cryopaste or Fraction I from human plasma; b) dissolving the Fraction I or cryopaste acquired in step a) in a pretreatment buffer, and conducting SID virus inactivation for enveloped virus; c) loading the treated solution from step b) to a canion chromatography; d) using cold ethanol precipitation to purify fibrinogen from the flow through in step c); e) loading elution buffer I to obtain factor VIII; f) dissolving a paste generated in step d) by using Buffer II for final formulation; and g) dialyzing and adding a stabilizer in the bulk acquired in step f).