Dendron-mediated DNA virus detection

摘要

The disclosure relates to chips containing nucleic acid probes or primers and their use in methods to detect nucleic acid molecules of DNA viruses. The disclosure includes DNA chips with probes immobilized via a dendron-mediated linkage in contact with a thermocycler capable of automatically regulating the temperature, temperature cycle times, and number of temperature cycles of the chips to provide genetic diagnosis in one step.

主权项

1. A method for detecting a papillomavirus nucleic acid molecule in a sample, said method comprising:
amplifying a papillomavirus nucleic acid molecule, in a biological sample, by use of a pair of primers in a polymerase chain reaction (PCR), to produce amplified nucleic acid material which is in a PCR reaction mix, in the presence of a single stranded nucleic acid probe that is complementary to said amplified nucleic acid material and is immobilized via attachment to the apex of a conical dendron that is attached to a solid surface by the base of said conical dendron, completing the last cycle of the PCR to produce amplified nucleic acid material, denaturing said amplified nucleic acid material in the presence of said PCR reaction mixture to form a denatured mixture in the presence of the immobilized probe and attached conical dendron, allowing the denatured amplified nucleic acid material, within the same PCR reaction mixture, to hybridize to said probe to form a double stranded complex in the presence of said denatured mixture, and detecting said complex attached to said solid surface.