Cell-based production of nonulosonates

摘要

The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose. Methods for producing the sugars are also provided.

主权项

1. A recombinant cell for the production of pseudaminic acid, comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding PseB enzyme function, a gene encoding PseC enzyme function, a gene encoding PseH enzyme function, a gene encoding PseI enzyme function, a gene encoding PseG enzyme function, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.