| 主权项 |
1. A process for inoculating a plurality of closed photobioreactors connected in parallel, each photobioreactor having a volume from about 10 liters to about 100 liters, with a cyanobacteria genetically enhanced for formation of a target molecule, comprising the steps of:
a. growing an inoculum culture of the genetically enhanced cyanobacteria in a first container to an OD750 of from about 1.0 to about 10.0 in a volume of from about 1 liter to about 10 liters of pre-sterilized medium; b. transferring the inoculum culture in a sterile manner under pressure without exposure to atmosphere from the first container to a pre-sterilized second container; c. growing the inoculum culture in the second container to an OD750 of from about 1.0 to about 10 in a volume of from about 20 liters to about 100 liters of pre-sterilized medium; d. transferring the inoculum culture in a sterile manner under pressure without exposure to atmosphere from the second container to a pre-sterilized third container; e. growing the inoculum culture in the third container to an OD750 of from about 2.0 to about 10 in a volume of from about 200 liters to about 1,000 liters of pre-sterilized medium; and f. transferring the inoculum culture in a sterile manner at a pressure of from about 5 to about 50 psi without exposure to atmosphere from the third container and a sterilized nutrient solution, to a plurality of pre-sterilized photobioreactors comprising a pre-sterilized liquid, wherein said pre-sterilized photobioreactors have inlet tubing comprising a drip emitter and are configured in parallel from the third container, wherein said drip emitter controls the culture flow so that each of the photobioreactors connected in parallel is inoculated at a substantially similar flow rate. |
