METHODS FOR MODULATING PROTEIN GLYCOSYLATION PROFILES OF RECOMBINANT PROTEIN THERAPEUTICS USING MONOSACCHARIDES AND OLIGOSACCHARIDES

摘要

The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of proteins expressed in host cells.

主权项

1. A method of producing a composition comprising a protein with a modulated glycosylation profile, said method comprising:
culturing a host cell expressing said protein in cell culture media supplemented with a monosaccharide and/or an oligosaccharide, thereby producing said composition comprising said protein with a modulated glycosylation profile as compared to a control, wherein said control is a composition comprising a protein produced by culturing a host cell expressing said protein in cell culture media which is not supplemented with a monosaccharide and/or an oligosaccharide.