METHOD FOR AMPLIFICATION AND ASSAY OF RNA FUSION GENE VARIANTS, METHOD OF DISTINGUISHING SAME AND RELATED PRIMERS, PROBES, AND KITS

摘要

Method for amplification, alone or in further combination with detection or detection and quantitation, of RNA from fusion gene variants, method of distinguishing same, and oligonucleotide primers and probes and kits for use in the methods.

主权项

1. A method of amplifying mRNA from variants of a fusion between a first gene and a second gene in a sample of mRNA, which method comprises:
(a) (i) obtaining cDNA, which has been reverse-transcribed from the sample of mRNA, or reverse-transcribing the sample of mRNA to cDNA and (ii) amplifying the reverse-transcribed cDNA using primers comprising a primer for an exon of the first gene, which becomes contiguous with a variant exon of the second gene, and a primer for each of two or more variant exons of the second gene, wherein one or more primers can be detectably labeled, whereupon mRNA from variants of a fusion between a first gene and a second gene in a sample of mRNA is amplified.