发明名称 |
DIAGNOSTICUM FOR DETERMINING THE PRESENCE OF TOTAL ENDOTOXIN (LIPOPOLYSACCHARIDE-LPS) OF GRAM-NEGATIVE BACTERIA AND ALSO THE GENUS AND TYPE OF GRAM-NEGATIVE BACTERIA PRODUCING ENDOTOXIN, METHOD FOR PRODUCING SAID DIAGNOSTICUM AND A SET |
摘要 |
<p>The invention relates to medicine, veterinary, and the food industry and can be used for endotoxin determination. The invention provides a diagnosticum, a method for producing it and a set for carrying out the abacterial instant diagnosis of the presence of gram-negative bacteria in a biological material, for determining the total LPS of endotoxin-producing gram-negative bacteria, and also for identifying the genus and type of endotoxin-producing gram-negative bacteria. In addition, said invention makes it possible to carry out for no more than 10 minutes the step-by-step instant diagnosis, starting with the detection of the presence of the total LPS of gram-negative bacteria to the detection of the LPS of the given genus and type of gram-negative bacteria. The inventive diagnosticum comprises a carrier in the form of a polystyrene latex with monoclonal antibodies sensitized thereon, said polystyrene latex carrier being represented by core-shell type particles, in which the core is the polystyrene latex and the shell is the styrene and zinc methacrylate copolymer at a mass unit ratio ranging from 1:0.8 to 1:0.4, and monoclonal antibodies, in particular of an IgG2a and IgG2b or IgG3 and IgG2a or IgG1 and IgG2a-k or IgG1 and IgG2a subclass, with which the latex particles are sensitized.</p> |
申请公布号 |
EP2204653(B1) |
申请公布日期 |
2014.09.17 |
申请号 |
EP20070872127 |
申请日期 |
2007.10.12 |
申请人 |
GOSUDARSTVENNOE UCHREZHDENIE NAUCHNY TSENTR SERDECHNO-SOSUDISTOI KHIRURGII IM. A.N. BAKULEVA ROSSIISKOI ACADEMII MEDITSINSKIKH NAUK;OBSCHESTVO S OGRANICHENNOI OTVETSTVENNOSTJU "NAUCHNO-PROIZVODSTVENNAYA FIRMA 'ROKHAT';BOKERIYA, LEONID ANTONOVICH;NIYAZMATOV, AGZAMDZHAN AKHTAMOVICH |
发明人 |
BOKERIYA, LEONID ANTONOVICH;NIYAZMATOV, AGZAMDZHAN AKHTAMOVICH |
分类号 |
G01N33/543;C12Q1/04;G01N33/569 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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