主权项 |
1. A method for determining a volume of ablated tissue comprising the steps of:
inserting an electrode into tissue, a longitudinal axis of the electrode defining a path of the electrode; supplying energy via the electrode to tissue to create an ablation volume therein; excising a plurality of slices of the tissue having at least a portion of the ablation volume therein, each of the plurality of slices having a thickness, a cross-sectional perimeter, and a trajectory point defined by the path of the electrode; subjecting the plurality of the slices to a first contrast agent for a predetermined period of time, wherein the contrast agent is configured to color dead cells within the ablation volume; rinsing the plurality of the slices with a rinse solution to remove any superficial attachment of the contrast agent from live cells of the tissue; drying the plurality of the slices to remove the rinse solution and the first contrast agent; determining a volume of each of the plurality of slices based on the trajectory point, the cross-sectional perimeter, and the thickness of each slice of the tissue; and summing the volumes from each of the plurality of slices to obtain the volume of ablated tissue. |