Synthetic 5'UTRs, expression vectors, and methods for increasing transgene expression

摘要

The present invention provides synthetic 5′UTRs comprising a first polynucleotide fragment and a second polynucleotide fragment, wherein the first polynucleotide fragment comprises at least one splice site of a first eukaryotic gene, the second polynucleotide fragment comprises at least a portion of 5′ untranslated region of a second eukaryotic gene, and the first polynucleotide fragment is located 5′ of the second polynucleotide fragment. In one embodiment, the first polynucleotide fragment comprises the second intron of a sarcoplasmic/endoplasmic reticulum calcium ATPase gene and the second polynucleotide fragment comprises at least a portion of the 5′ untranslated region (5′UTR) of a eukaryotic casein gene. The synthetic 5′UTRs are useful for increasing the expression of a transgene when positioned between a promoter and a transgene within an expression vector. The present invention also provides vectors comprising synthetic 5′UTRs and methods for increasing the expression of a transgene using synthetic 5′UTRs.

主权项

1. A polynucleotide construct comprising: a first polynucleotide comprising one of SEQ ID NOS:2 and 4-6, a second polynucleotide comprising one of SEQ ID NOS:3 and 8-10, a third polynucleotide encoding a sequence of interest to be expressed in a cell, wherein the first polynucleotide is located 5′ of the second polynucleotide.