Method for counting colonies

摘要

Colonies growing on a culture medium surface may be more accurately counted with reduced variation in the counts obtained by capturing a plurality of images of the entire culture medium surface, wherein successive images are captured following rotation of the culture medium surface relative to the image capture apparatus by n/360 degrees where n is the number of images to be captured; performing data processing on image data of the entire culture medium surface of the petri dish at each specified angle; and the number of colonies in the petri dish is calculated by performing numerical processing on the number of colonies counted separately at each specified angle.

主权项

1. A process for using an imaging apparatus to count the number of colonies on a culture medium surface comprising the steps of:
positioning a petri dish having a culture medium surface and a colony to be counted in an imaging apparatus; imaging the entire culture medium surface of the petri dish using the imaging apparatus; rotating the petri dish around a rotational axis within the imaging apparatus by 360/n degrees, where n is a positive integer; imaging the entire culture medium surface of the petri dish at that rotational position; repeating the rotating and imaging steps n−1 times; processing the image data of the entire culture medium surface of the petri dish to count the number of colonies, wherein a counting region for the counting the number of colonies is determined to be the whole petri dish inside the side wall, and the number of colonies in the counting region at a specified angle is counted and acquired; acquiring a colony count value for each of the n entire culture medium surface images; and combining and averaging the number of colonies counted for each of the entire culture medium surface images in the petri dish to obtain a colony count for the culture medium surface within the petri dish.