Compositions and methods to control insect pests

摘要

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

主权项

1. An isolated polynucleotide comprising a nucleotide sequence comprising:
(a) the nucleotide sequence comprising SEQ ID NO: 8, or a complement thereof; (b) the nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO: 8, or a complement thereof; wherein said polynucleotide encodes a silencing element having insecticidal activity against a Coleopteran plant pest; or (c) the nucleotide sequence comprising at least 19 consecutive nucleotides of SEQ ID NO: 8, or a complement thereof; wherein said polynucleotide encodes a silencing element having insecticidal activity against a Coleopteran plant pest.