REVERSIBLE TERMINATOR MOLECULES AND METHODS OF THEIR USE

摘要

The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3′ hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.

主权项

1. One or more reversible terminator compound selected from the group consisting of: wherein R is optionally substituted alkyl of C1-C6, or an optionally substituted aryl, X is O, S, or BH3, B is an optionally substituted heterocyclic nucleic acid base, or an analog thereof, L is an optional linker group which is optionally cleavable, and D1 and D2 are optional detectable labels.