HIGH-THROUGHPUT SYSTEM AND METHOD FOR IDENTIFYING ANTIBODIES HAVING SPECIFIC ANTIGEN BINDING ACTIVITIES

摘要

System and methods are disclosed for identifying and isolating antibodies with specific affinity with an antigen of interest. Multiple DNA libraries encoding antibodies or their fragments are designed such that the encoded antibodies from different libraries are tagged differently. These libraries may be transformed into yeast cells. The variants of the antibodies are displayed on the surface of the yeast cells and flow cytometry may be used to sort the cells based on antigen affinity and the different tags on the antibodies. By allowing multiple libraries to be screened simultaneously, the disclosed methods help improve the efficiency of affinity.

主权项

1. A method of selecting binding proteins that specifically binds to a target comprising
a) transfecting nucleic acids from a first nucleic acid library, a second nucleic acid library and a third nucleic acid library into host cells of an organism, wherein the nucleic acids encode binding proteins, wherein the nucleic acids in each nucleic acid library comprise a variable region in distinct regions of the nucleic acid molecules and wherein the nucleic acid molecules of each library encode a distinct tag; b) expressing the binding proteins encoded by the nucleic acid molecules on the surface of the host cells; c) exposing the host cells in each library to the target; and d) selecting host cells expressing binding proteins that specifically bind to the target.