Diagnosis and treatment of kidney stones, methods and compositions therefor

摘要

Methods of detecting, diagnosing, monitoring and treating kidney stones are disclosed. In some embodiments, methods of detecting, diagnosing or monitoring kidney stones comprise contacting a urine sample with anti-Claudin-14 antibody, and detecting quantity of a complex comprising Claudin-14 and the antibody, wherein an increase compared to control levels is diagnostic for kidney stones. In some embodiments, methods further comprise testing a second sample at a second time point to detect increased kidney stones. In some embodiments, methods of treating kidney stone disease comprise administering an miR-9 mimic or an miR-374 mimic. In some embodiments, methods comprise administering an inhibitor of CaSR signaling. In some embodiments, methods comprise administering a HDAC inhibitor. In some embodiments methods of treating hyperparathyroidism and hypercalcemia are disclosed, comprising administering an agonist of CaSR. Methods of abrogating CaSR-mediated regulation of claudin-14, microRNAs and urinary Ca++ excretion are disclosed, comprising administering a calcineurin inhibitor.

主权项

1. A method of detecting, diagnosing or monitoring kidney stone disease in a subject, comprising:
providing a urine sample from a subject having or suspected of having kidney stone disease; contacting the sample with an antibody that binds Claudin-14 polypeptide, under conditions sufficient for formation of a primary complex comprising the antibody and the Claudin-14 polypeptide if present; measuring quantity of the primary complex; comparing the quantity of the primary complex to that of a control complex formed from the antibody and a urine sample of an individual who does not have kidney stone disease; and detecting kidney stone disease if the quantity of the primary complex from the subject is statistically significantly greater than that of an individual who does not have kidney stone disease.