Substrate for forming a three-dimensional tissue construct and method of use

摘要

The present invention provides a tissue construct-forming substrate for forming a three-dimensional tissue construct containing proliferating cells, the substrate including a porous film having through-holes, and the porous film having, on the surface of the film, a cell adhesive region capable of retaining cells and a cell non-adhesive region located at a peripheral region of the cell adhesive region, a tissue construct-forming kit comprising the above-mentioned tissue construct-forming substrate and a frame, and a method for forming the above-mentioned tissue construct.

主权项

1. A tissue construct-forming substrate, the substrate comprising a porous film having through-holes, and the porous film having, on each of a front surface and a back surface of the film, a plurality of cell adhesive regions capable of retaining cells and a cell non-adhesive region located at a peripheral region of each of the cell adhesive regions;
wherein the cell non-adhesive regions respectively form a continuous region, the porous film comprises the plural cell adhesive regions disposed such that adjacent cell adhesive regions are spaced a predetermined distance apart, and at least one of the through-holes is a transmission path that transfers a substance between the cell non-adhesive region on the front surface of the porous film and the cell non-adhesive region on the back surface of the porous film.