发明名称 |
MULTIVALENT BINDING PROTEIN COMPOSITIONS AND METHODS FOR IDENTIFYING VARIANTS OF SAME |
摘要 |
Provided are protein, nucleic acid, and cellular libraries of multivalent binding proteins (e.g., DVD-Fab or DVD-Ig molecules) and the use of these libraries for the screening of multivalent binding proteins using cell surface display technology (e.g., yeast display). |
申请公布号 |
US2014235476(A1) |
申请公布日期 |
2014.08.21 |
申请号 |
US201314141501 |
申请日期 |
2013.12.27 |
申请人 |
AbbVie, Inc. |
发明人 |
GU Jijie;HARRIS Maria Cristina;BENATUIL Lorenzo;HSIEH Chung-Ming |
分类号 |
G01N33/566;C07K16/18 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
1. A diverse library of binding proteins comprising a first polypeptide chain having the general formula VH1-(X1)n-VH2-C—(X2)n, wherein VH1 is a first heavy chain variable domain, X1 is a linker with the proviso that it is not a constant domain, VH2 is a second heavy chain variable domain, C is a heavy chain constant domain, X2 is a cell surface protein, and n is 0 or 1, and wherein the amino acid sequences of VH1, VH2 and/or X1 independently vary within the library. |
地址 |
Worcester MA US |