Low Numerical Aperture Exclusion Imaging

摘要

In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography. Optical sectioning provides the information necessary for 3D image reconstructions and other X Axis spatial measurements.

主权项

1. A low numerical aperture exclusion imaging apparatus, comprising:
an electromagnetic illumination source for illuminating a portion of a specimen; an objective lens optically coupled to the electromagnetic illuminated portion of the specimen, for collecting an image created by the electromagnetic radiation illuminating a portion of the specimen; an optical blocking plate, wherein the optical blocking plate is positioned to substantially block electromagnetic radiation transmitted through a center portion of the objective lens, and wherein the optical blocking plate and the objective lens are symmetric about a common axis.