DEVICE AND METHOD FOR HIGH-PRESSURE CRYOPRESERVATION OF A BIOLOGICAL SAMPLE

摘要

A cryopreservation device (100) which is arranged for cryopreservation of a biological sample (1) comprises a pressure vessel (10) with a vessel wall (11) and an internal space (12) which is arranged to receive the biological sample (1), wherein the pressure vessel (10) is equipped with an actuating device (20) for cooling by lowering the temperature and raising the pressure in the pressure vessel (10) and configured for the cryopreservation of the biological sample (1). Said actuating device (20) is connected to the vessel wall (11) and comprises at least one pressure setting element (21-23, 31) and at least one of at least one cooling element (34) and at least one heat conducting element (35-38), wherein the actuating device (20) is configured for a time-dependent and/or location-dependent setting of the temperature and of the pressure in the pressure vessel (10). Methods are also described for cryopreservation of a biological sample (1), comprising biological cells (2) and a preservation medium (3), and methods for heating the biological sample (1) to maintain vitality.