| 摘要 |
The object is to provide a cassette for fixing, embedding and slicing biological tissues which is made of a sliceable material, which makes it possible to place biological tissues in this cassette, and fix, embed and slice the tissues in this same cassette, thereby simplifying the processing steps and saving labor, and also eliminating the possibility of deformation and crushing of the tissues, and the possibility of misplacement of the tissues, and which makes it possible to obtain accurate information on examination of biological tissues, and to provide a method of fixing and embedding this cassette to form a block, and slicing this block. The cassette 10A for fixing, embedding and slicing biological tissues includes a base sheet 11' and a lid sheet 12' formed of an elongated plastic sheet which is sliceable and has not less than predetermined strength. The base sheet 11' has grooves 13 as biological tissue receiving portions. The base plate 11' and the lid sheet 12' are formed by folding the plastic sheet along a fold line 14 so that the lid sheet 12' can be selectively opened and closed. Biological tissues are pressed against the bottoms of the grooves with pressing/retaining sponge members 13a secured to the lid sheet 12' at its portions corresponding to the respective grooves. In this state, the biological tissues are fixed by a fixing agent, and the cassette is dehydrated, infiltrated with paraffin as an embedding agent to embed the cassette, thereby forming a block including an embedding frame. The block is then sliced with a microtome to provide good biological tissue specimens. |
