Detection of feline immunodeficiency virus

摘要

Method, device and kit for the detection of antibodies directed to Feline Immunodeficiency Virus (FIV). The method includes contacting the felid biological sample with FIV env polypeptide and detecting whether the polypeptide substantially binds to the antibody in the biological sample. The method will detect FIV antibodies in a sample from animals that have been naturally infected but the method will not detect antibodies in a sample from animals that have not been infected and that have not been vaccinated with an FIV vaccine after within about the previous five to eight weeks.

主权项

1. A method for detecting antibodies to Feline Immunodeficiency Virus (FIV) in a biological sample, the method comprising:
(a) contacting a biological sample from a felid having an unknown vaccination and HIV infection status with the FIV Env polypeptide of SEQ ID NO: 1 (ELGCNQNQFFCK) and a monoclonal antibody that binds to SEQ ID NO: 1, (b) detecting whether antibodies in the sample compete with the monoclonal antibody for binding to SEQ ID NO: 1, thereby determining that the sample contains antibodies to FIV, wherein the FIV antibodies in a sample from felids that have been naturally infected will compete with the monoclonal antibody for binding to SEQ ID NO: 1 but antibodies in a sample from felids that have not been infected or felids that have been vaccinated with a whole killed FIV vaccine more than five to eight weeks prior to the sample being obtained will not compete with the monoclonal antibody for binding to SEQ ID NO: 1; and (c) repeating steps (a) and (b) for a second biological sample obtained after about five to eight weeks from the same felid if antibodies in the first biological sample compete with the monoclonal antibody for binding to SEQ ID NO:1 in step (b), wherein the felid is naturally infected if antibodies in the second sample compete with the monoclonal antibody for binding to SEQ ID NO: 1, or the felid has not been infected and has been vaccinated with a whole killed FIV vaccine within about five to eight weeks prior to the first biological sample being obtained if antibodies in the second sample do not compete with the monoclonal antibody for binding to SEQ ID NO: 1.