Method and system for microfluidic device and imaging thereof

摘要

A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

主权项

1. A method of processing a deformable microfluidic device having a plurality of chambers each capable of holding a fluid therein and having a plurality of fiducial marks, the method comprising:
identifying at least three fiducial marks of the plurality of fiducial marks that are disposed within a vicinity of one or more chambers of the plurality of chambers; thereafter capturing an image of the at least three fiducial marks, where each of the at least three fiducial marks correspond to a design location in an undeformed state of the deformable microfluidic device, and the design locations have a first spatial relationship with respect to each other in a design space, and where each of the at least three fiducial marks are disposed at actual locations in a deformed state of the deformable microfluidic device, and at least one of the at least three fiducial marks has an actual location that is shifted from a corresponding design location such that the actual locations of the at least three fiducial marks have a second spatial relationship with respect to each other in a measurement space that is different from the first spatial relationship; and generating a mapping between the design space and the measurement space based on the image.