Modification of plant lignin content

摘要

DNA constructs comprising a first DNA segment that corresponds to at least a portion of a gene in the monolignol biosynthetic pathway, a spacer DNA segment, and a second DNA segment that is complementary to the first DNA segment can be used to reduce or modulate the lignin content in plants. In some embodiments, DNA constructs comprise at least a portion of a gene for 4CL, C3H, CCR, C4H or CCoAOMT. Vascular-preferred and constitutive promoters can be used to drive expression of the constructs.

主权项

1. A DNA construct comprising a promoter operably linked to a first DNA segment comprising at least 50 bp of a Eucalyptus grandis cinnamate 4-hydroxylase (C4H) gene sequence disclosed in SEQ ID NO: 47, an intron spacer DNA segment comprising SEQ ID NO: 9, and a second DNA segment comprising a Eucalyptus grandis cinnamate 4-hydroxylase (C4H) gene sequence disclosed in SEQ ID NO: 47 that is fully complementary to the first DNA segment, wherein the first and second DNA segments are arranged in a 5′ to 3′ direction with respect to each other.