Method of preparing a glycoside of a mono- or diacylglycerol product from a plant material

摘要

The present invention provides a method of preparing a glycoside of a mono- or diacylglycerol product from a plant material. The method comprises the steps of: (i) optionally milling the plant material, (ii) extracting the optionally milled plant material with a first aqueous extraction solution obtaining a first liquid phase and a first solid phase, (iii) separating the liquid phase from the solid phase to obtain a glycoside of mono- or diacylglycerol product, (iv) extracting the solid phase from step (iii) with a second extraction solution obtaining a second liquid phase and a second phase, and (v) separating the second liquid phase from the second solid phase to obtain a second and a third glycoside of mono- or diacylglycerol product, wherein the second extraction solution further comprises a cell wall degrading enzyme or a mixture of cell wall degrading enzymes.

主权项

1. A method of obtaining a product containing at least one glycoside of a mono- or diacylglycerol selected from the group consisting of 3 β-D-galactopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3β-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-galactopyranosyloxy-2-(octadeca-9Z,I2Z,I5Z trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, and mixtures thereof from a plant material,
said method consisting of the following steps, taken in sequence, of: obtaining a plant material which contains at least one glycoside of a mono- or diacylglycerol selected from the group consisting of 3 β-D-galactopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3β-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-galactopyranosyloxy-2-(octadeca-9Z,I2Z,I5Z trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, and mixtures thereof; (i) optionally milling the plant material, (ii) extracting the optionally milled plant material with water and producing a first liquid phase and a first solid phase which contains the at least one glycoside of a mono- or diacylglycerol; (iii) separating the first liquid phase from the first solid phase, wherein the separated first liquid phase comprises a liquid product containing a glycoside of mono- or diacylglycerol, (iv) extracting the first solid phase obtained from step (iii) with water and producing a second liquid phase and a second solid phase which contains the at least one glycoside of a mono- or diacylglycerol, and, (v) separating the second liquid phase from the second solid phase to obtain in the second solid phase a solid concentrate product containing a concentrate of the at least one glycoside of a mono- or diacylglycerol selected from the group consisting of 3 β-D-galactopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3β-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-galactopyranosyloxy-2-(octadeca-9Z,I2Z,I5Z trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, 3-α-D-glucopyranosyloxy-2-(octadeca-9Z,12Z,15Z-trienoyloxy)propanyl octadeca-9Z,12Z,15Z-trienoate, and mixtures thereof, and, wherein no organic solvents are used in any of steps i-v.