Methods of cell culture for adoptive cell therapy

摘要

An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.

主权项

1. An improved method of producing cells comprising:
growing activated antigen-specific T cells in the presence of irradiated antigen-presenting cells-on a growth surface and with a medium volume to surface area ratio of up to 1 ml/cm2; wherein said surface density of the antigen-specific T cells plus the surface density of said antigen presenting cells is between 128,906 and 1,125,000 cells/cm2 and the surface density of the antigen-specific T cells is less than 250,000 cells/cm2 and the combined number of said antigen presenting cells and antigen-specific T cells is less than 500,000 cells/cm2 at the onset of a production cycle.