METHODS AND KITS USED IN IDENTIFYING MICRORNA TARGETS

摘要

Described herein are methods and kits used to identify an endogenously expressed target mRNA of a microRNA of interest. The method involves the use of a dominant negative GW182 polypeptide that forms a stable complex with the target mRNA. The method further involves purifying the complex and identifying the target mRNA.

主权项

1. A method of identifying a target mRNA of a microRNA of interest, the method comprising:
contacting the microRNA of interest with a dominant negative GW182 polypeptide within a cell; purifying a complex comprising the dominant negative GW182 polypeptide and an endogenously expressed target mRNA of the microRNA of interest; and identifying the endogenously expressed target mRNA.