RAPID AND SIMULTANEOUS DETECTING METHOD DEVELOPMENT OF STEROIDS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

A rapid and simultaneous detection method for steroids according to the present invention includes a step for pre-treating a sample contained in a matrix; a step for measuring characteristics of the pre-treated sample by using high-performance liquid chromatography; a step for selecting a retention time (RT) of a peak and a spectrum with respect to the peak among the measured characteristics as sample detection keys; a step for searching a steroid satisfying a parity condition equal to or above the search key and a predetermined value through comparison with a library in a steroid database; and a step for outputting the presence or absence of the steroid satisfying the parity condition and the type thereof based on the search result. When this method is used, inclusion of various types of steroids in an analysis target sample and the types thereof can be quickly searched even without an expensive mass analysis device.